Review



goat anti-darpp32  (Absolute Biotech Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Absolute Biotech Inc goat anti-darpp32
    a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. <t>DARPP32</t> and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.
    Goat Anti Darpp32, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti-darpp32/product/Absolute Biotech Inc
    Average 90 stars, based on 1 article reviews
    goat anti-darpp32 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease"

    Article Title: Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease

    Journal: bioRxiv

    doi: 10.1101/2023.02.23.529222

    a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.
    Figure Legend Snippet: a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.

    Techniques Used: Western Blot, Two Tailed Test, Immunofluorescence, Fluorescence, Expressing



    Similar Products

    anti darpp32 goat polyclonal antibody  (R&D Systems)
    94
    R&D Systems anti darpp32 goat polyclonal antibody
    Anti Darpp32 Goat Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti darpp32 goat polyclonal antibody/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    anti darpp32 goat polyclonal antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    goat anti-darpp32  (Absolute Biotech Inc)
    90
    Absolute Biotech Inc goat anti-darpp32
    a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. <t>DARPP32</t> and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.
    Goat Anti Darpp32, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti-darpp32/product/Absolute Biotech Inc
    Average 90 stars, based on 1 article reviews
    goat anti-darpp32 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    goat anti-darpp32  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology goat anti-darpp32
    a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. <t>DARPP32</t> and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.
    Goat Anti Darpp32, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti-darpp32/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    goat anti-darpp32 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    goat anti-mouse darpp32 antibody  (Millipore)
    90
    Millipore goat anti-mouse darpp32 antibody
    Selective accumulation of mhtt aggregates in MSNs in the striatal model . (A) Double immunofluorescent staining with EM48 antibody [30] and <t>anti-Darpp32</t> (a MSN marker) using 6 month old coronal brain sections from striatal, cortical, and pan-neuronal models of HD. In both striatal and pan-neuronal models the vast majority of MSNs (>98%) have diffuse nuclear EM48 staining. Such a staining pattern is not present in the MSNs in the cortical model or WT mice at this age (data not shown for the WT). (B). Double immunofluorescent staining with EM48 antibody and anti-GABA antibody (marker for cortical interneurons) in the striatal model of HD reveal that a few cells that accumulate nuclear EM48 staining in the cortex are GABA (+) interneurons (white arrows). Scale bars: 100 μm
    Goat Anti Mouse Darpp32 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti-mouse darpp32 antibody/product/Millipore
    Average 90 stars, based on 1 article reviews
    goat anti-mouse darpp32 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    goat anti mouse darpp32  (R&D Systems)
    94
    R&D Systems goat anti mouse darpp32
    (A) Photomicrographs of the dorsal striatum showing levels of SPOTlight EGFP and tdTomato (amplified by anti-RFP/Rhodamine Red) in CINs (ChAT+, top) and SPNs <t>(DARPP32+,</t> bottom). (B) Phospho-eIF2α immunofluorescence in CINs (top) and SPNs (bottom). (C) Quantification of p-eIF2α CTCF from (B). Images were analyzed with FIJI, and immunofluorescence was determined per cell and presented as the mean CTCF ± SEM. (ChAT+ - 3 mice, 92 cells; DARPP32+ - 3 mice, 395 cells). Nested t-test, t=12.54, F(1,4)=157.3, ***p=0.0002. Scale bar 25 microns. (D) Strategy for Cre-mediated expression of hM4Di (Gi) DREADD fused to mCherry or mCherry alone in dorsal striatal CINs of ChAT-Cre heterozygous mice. All mice received chronic clozapine-N-oxide (CNO) at a dose of 3 mg/kg/day divided in two evenly spaced doses for 5 days. (E) Representative photomicrographs from the dorsal striatum of mCherry control (top) vs Gi DREADD-expressing (bottom) mice showing ChAT immunohistochemistry, mCherry expression, p-eIF2α immunohistochemistry with DAPI counterstain, and merged images. Scale bar 25 microns. (F) Quantification of p-eIF2α CTCF from (E). (mCherry control - 5 mice, 270 ChAT+ cells; Gi DREADD - 5 mice, 215 ChAT+ cells). Nested t-test: t=4.079, F(1,8)=16.64, **p=0.0035.
    Goat Anti Mouse Darpp32, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse darpp32/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    goat anti mouse darpp32 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    goat polyclonal thr34 darpp32  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology goat polyclonal thr34 darpp32
    Prelimbic mPFC and Orbitofrontal cortex. Intensity and Ratio values across brain regions and conditions.
    Goat Polyclonal Thr34 Darpp32, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal thr34 darpp32/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    goat polyclonal thr34 darpp32 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.

    Journal: bioRxiv

    Article Title: Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease

    doi: 10.1101/2023.02.23.529222

    Figure Lengend Snippet: a , Western blots of the indicated brain regions from 8-week-old R6/2 mice and WT littermate controls. HDGF band is indicated on the right, the upper band is unspecific. Total protein detection was used as loading control. b , Quantification of HDGF protein quantity in the indicated brain regions of 8- and 12-week-old R6/2 mice, normalized first to total protein and then to the average value of the littermate controls. N=4-5 mice per group. Unpaired two-tailed t-test, per brain region and age group. No significant differences were observed. CE, cerebellum; HP, hippocampus; CX, cortex; ST, striatum. c , Images of brain sections from 12-week-old R6/2 and WT mice immunostained for HDGF. DARPP32 and neurogranin (NGRN) were used as markers of striatal MSNs and cortical PCs, respectively. Nuclei were counterstained with DAPI. d , Quantification of HDGF immunofluorescence intensity in striatal MSNs and cortical PCs of R6/2 mice and WT controls. Values were background-subtracted and normalized to the fluorescence intensity of the highest expressing cell in the field of view. N=3 mice per group. Unpaired two-tailed t-test, per cell type. No significant differences were observed. Scale bar in c, 20 µm.

    Article Snippet: The following primary antibodies were used: rabbit anti-HDGF (Abcam, ab128921, 1:500), mouse anti-HTT (EM48, Chemicon, MAB5374, 1:500), mouse anti-Flag (Origene, TA50011, 1:1,000), goat anti-ChAT (Chemicon, AB144, 1:500), goat anti-DARPP32 (LifeSpan Biosciences, LS-C150127, 1:300), mouse anti-NGRN (R&D Systems, MAB7947, 1:60), chicken anti-GFAP (Origene, AP31806PU-N, 1:2,000, with antigen retrieval), mouse anti-APC (Calbiochem, OP80, 1:20, with antigen retrieval), and goat anti-IBA1 (Abcam, ab107159, 1:1,000, with antigen retrieval).

    Techniques: Western Blot, Two Tailed Test, Immunofluorescence, Fluorescence, Expressing

    Selective accumulation of mhtt aggregates in MSNs in the striatal model . (A) Double immunofluorescent staining with EM48 antibody [30] and anti-Darpp32 (a MSN marker) using 6 month old coronal brain sections from striatal, cortical, and pan-neuronal models of HD. In both striatal and pan-neuronal models the vast majority of MSNs (>98%) have diffuse nuclear EM48 staining. Such a staining pattern is not present in the MSNs in the cortical model or WT mice at this age (data not shown for the WT). (B). Double immunofluorescent staining with EM48 antibody and anti-GABA antibody (marker for cortical interneurons) in the striatal model of HD reveal that a few cells that accumulate nuclear EM48 staining in the cortex are GABA (+) interneurons (white arrows). Scale bars: 100 μm

    Journal: Molecular Neurodegeneration

    Article Title: Pathological cell-cell interactions are necessary for striatal pathogenesis in a conditional mouse model of Huntington's disease

    doi: 10.1186/1750-1326-2-8

    Figure Lengend Snippet: Selective accumulation of mhtt aggregates in MSNs in the striatal model . (A) Double immunofluorescent staining with EM48 antibody [30] and anti-Darpp32 (a MSN marker) using 6 month old coronal brain sections from striatal, cortical, and pan-neuronal models of HD. In both striatal and pan-neuronal models the vast majority of MSNs (>98%) have diffuse nuclear EM48 staining. Such a staining pattern is not present in the MSNs in the cortical model or WT mice at this age (data not shown for the WT). (B). Double immunofluorescent staining with EM48 antibody and anti-GABA antibody (marker for cortical interneurons) in the striatal model of HD reveal that a few cells that accumulate nuclear EM48 staining in the cortex are GABA (+) interneurons (white arrows). Scale bars: 100 μm

    Article Snippet: The following primary antibodies were used: rabbit anti-human htt EM48 antibody (1:300), mouse anti-GFAP antibody (1:10000, Sigma), goat anti-mouse Darpp32 antibody (1:1000, Chemicon), mouse anti-GABA (1: 1000, Sigma).

    Techniques: Staining, Marker

    (A) Photomicrographs of the dorsal striatum showing levels of SPOTlight EGFP and tdTomato (amplified by anti-RFP/Rhodamine Red) in CINs (ChAT+, top) and SPNs (DARPP32+, bottom). (B) Phospho-eIF2α immunofluorescence in CINs (top) and SPNs (bottom). (C) Quantification of p-eIF2α CTCF from (B). Images were analyzed with FIJI, and immunofluorescence was determined per cell and presented as the mean CTCF ± SEM. (ChAT+ - 3 mice, 92 cells; DARPP32+ - 3 mice, 395 cells). Nested t-test, t=12.54, F(1,4)=157.3, ***p=0.0002. Scale bar 25 microns. (D) Strategy for Cre-mediated expression of hM4Di (Gi) DREADD fused to mCherry or mCherry alone in dorsal striatal CINs of ChAT-Cre heterozygous mice. All mice received chronic clozapine-N-oxide (CNO) at a dose of 3 mg/kg/day divided in two evenly spaced doses for 5 days. (E) Representative photomicrographs from the dorsal striatum of mCherry control (top) vs Gi DREADD-expressing (bottom) mice showing ChAT immunohistochemistry, mCherry expression, p-eIF2α immunohistochemistry with DAPI counterstain, and merged images. Scale bar 25 microns. (F) Quantification of p-eIF2α CTCF from (E). (mCherry control - 5 mice, 270 ChAT+ cells; Gi DREADD - 5 mice, 215 ChAT+ cells). Nested t-test: t=4.079, F(1,8)=16.64, **p=0.0035.

    Journal: Science (New York, N.Y.)

    Article Title: Cholinergic neurons engage the integrated stress response for dopamine modulation and skill learning

    doi: 10.1126/science.abe1931

    Figure Lengend Snippet: (A) Photomicrographs of the dorsal striatum showing levels of SPOTlight EGFP and tdTomato (amplified by anti-RFP/Rhodamine Red) in CINs (ChAT+, top) and SPNs (DARPP32+, bottom). (B) Phospho-eIF2α immunofluorescence in CINs (top) and SPNs (bottom). (C) Quantification of p-eIF2α CTCF from (B). Images were analyzed with FIJI, and immunofluorescence was determined per cell and presented as the mean CTCF ± SEM. (ChAT+ - 3 mice, 92 cells; DARPP32+ - 3 mice, 395 cells). Nested t-test, t=12.54, F(1,4)=157.3, ***p=0.0002. Scale bar 25 microns. (D) Strategy for Cre-mediated expression of hM4Di (Gi) DREADD fused to mCherry or mCherry alone in dorsal striatal CINs of ChAT-Cre heterozygous mice. All mice received chronic clozapine-N-oxide (CNO) at a dose of 3 mg/kg/day divided in two evenly spaced doses for 5 days. (E) Representative photomicrographs from the dorsal striatum of mCherry control (top) vs Gi DREADD-expressing (bottom) mice showing ChAT immunohistochemistry, mCherry expression, p-eIF2α immunohistochemistry with DAPI counterstain, and merged images. Scale bar 25 microns. (F) Quantification of p-eIF2α CTCF from (E). (mCherry control - 5 mice, 270 ChAT+ cells; Gi DREADD - 5 mice, 215 ChAT+ cells). Nested t-test: t=4.079, F(1,8)=16.64, **p=0.0035.

    Article Snippet: Commercially available primary antibodies to rabbit anti-mouse p-eIF2alpha (1:100, Abcam, cat# ab32157; RRID: AB_732117), goat anti-mouse Choline acetyltransferase (ChAT) (1:200, Millipore, cat# AB144P; RRID: AB_2079751), chicken anti-mouse Tyrosine Hydroxylase (TH) (1:500, Abcam cat# ab76442; RRID: AB_1524535), goat anti-mouse DARPP32 (R&D Systems, cat# AF6259; RRID: AB_10641854), anti-Myc tag (1:200, Abcam, cat# ab9106; RRID: AB_307014), rabbit anti-mouse p-PERK (Thr980) (1:1000, Bioss, cat#Bs-3330R; RRID: AB_10855345), and rabbit anti-mouse RFP (1:1000, Rockland antibodies and assays, cat# 600–401-379; RRID: AB_2209751) or chicken anti-mouse RFP (1:500, Novus Biologicals, cat# NBP1–97371; RRID: AB_11139267) were used for these experiments.

    Techniques: Amplification, Immunofluorescence, Expressing, Control, Immunohistochemistry

    Prelimbic mPFC and Orbitofrontal cortex. Intensity and Ratio values across brain regions and conditions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: Prelimbic mPFC and Orbitofrontal cortex. Intensity and Ratio values across brain regions and conditions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    VTA. Intensity and Ratio values across conditions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: VTA. Intensity and Ratio values across conditions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    DARPP32 in the prelimbic area of the mPFC. Panel (A) depicts pThr34 DARPP32 alone and ( B ), the phosphorylation of DARPP32 at Thr34 as a ratio value. *indicates statistically significant from NO TEST, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in the prelimbic area of the mPFC. Panel (A) depicts pThr34 DARPP32 alone and ( B ), the phosphorylation of DARPP32 at Thr34 as a ratio value. *indicates statistically significant from NO TEST, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    DARPP32 in VTA. Panel (A) depicts DARPP32 in all subjects, comparing Days 1 and 30 of abstinence; Panel (B) compares phosphorylation of DARPP32 at Thr34 as a ratio value on Days 1 and 30 of abstinence. *indicates statistically significant from Day 1, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in VTA. Panel (A) depicts DARPP32 in all subjects, comparing Days 1 and 30 of abstinence; Panel (B) compares phosphorylation of DARPP32 at Thr34 as a ratio value on Days 1 and 30 of abstinence. *indicates statistically significant from Day 1, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    DARPP32 in the OFC. Panel (A) depicts pThr34 DARPP32 in tested subjects across all other experimental conditions; ( B ) depicts phosphorylation of DARPP32 at Thr34 as a ratio value for subjects comparing Days 1 and 30 of abstinence. *indicates statistically different from Day 1 condition, p < 0.05. # indicates statistically significant from CON Day 30, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in the OFC. Panel (A) depicts pThr34 DARPP32 in tested subjects across all other experimental conditions; ( B ) depicts phosphorylation of DARPP32 at Thr34 as a ratio value for subjects comparing Days 1 and 30 of abstinence. *indicates statistically different from Day 1 condition, p < 0.05. # indicates statistically significant from CON Day 30, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    Infralimbic and Anterior Cingulate mPFC. Intensity and Ratio values across brain regions and conditions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: Infralimbic and Anterior Cingulate mPFC. Intensity and Ratio values across brain regions and conditions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    DARPP32 in infralimbic area of mPFC. Panel (A) depicts pThr34 in all rats (not-tested and tested) in all experimental conditions; panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value, also in all rats (not-tested and tested) in all experimental conditions. *indicates statistically significant difference(s), p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in infralimbic area of mPFC. Panel (A) depicts pThr34 in all rats (not-tested and tested) in all experimental conditions; panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value, also in all rats (not-tested and tested) in all experimental conditions. *indicates statistically significant difference(s), p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    Dorsal Striatal regions. Intensity and Ratio values across brain regions and conditions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: Dorsal Striatal regions. Intensity and Ratio values across brain regions and conditions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    DARPP32 in DMS. Panel (A) depicts DARPP32 in tested subjects in all experimental conditions; panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value in rats that were not tested, but also in all experimental conditions. *indicates statistically significant from CON Day 1, p < 0.05. # indicates statistically significant from CON Day 30, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in DMS. Panel (A) depicts DARPP32 in tested subjects in all experimental conditions; panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value in rats that were not tested, but also in all experimental conditions. *indicates statistically significant from CON Day 1, p < 0.05. # indicates statistically significant from CON Day 30, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    DARPP32 in DMS (cont.) and in DLS. Panel (A) depicts pThr34 DARPP32 in all subjects across housing conditions; Panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value in all rats comparing Days 1 and 30 of abstinence. *indicates statistically significant from Day 1, p < 0.05. † indicates statistically significant from EEAcute, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in DMS (cont.) and in DLS. Panel (A) depicts pThr34 DARPP32 in all subjects across housing conditions; Panel (B) depicts phosphorylation of DARPP32 at Thr34 as a ratio value in all rats comparing Days 1 and 30 of abstinence. *indicates statistically significant from Day 1, p < 0.05. † indicates statistically significant from EEAcute, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    Ventral Striatal regions. Intensity and Ratio values across brain regions and conditions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: Ventral Striatal regions. Intensity and Ratio values across brain regions and conditions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    DARPP32 in nucleus accumbens core. Panel (A) depicts DARPP32 in all subjects in all housing conditions; panel (B) represents these same groupings, but depicts phosphorylation of DARPP32 at Thr34 as a ratio value. *indicates statistically significant from CON, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in nucleus accumbens core. Panel (A) depicts DARPP32 in all subjects in all housing conditions; panel (B) represents these same groupings, but depicts phosphorylation of DARPP32 at Thr34 as a ratio value. *indicates statistically significant from CON, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: Phospho-proteomics

    DARPP32 in nucleus accumbens shell. DARPP32 in subjects that were not tested, but in all other experimental conditions. *indicates statistically significant from CON Day 1, p < 0.05. Representative blots for each condition are provided.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: DARPP32 in nucleus accumbens shell. DARPP32 in subjects that were not tested, but in all other experimental conditions. *indicates statistically significant from CON Day 1, p < 0.05. Representative blots for each condition are provided.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques:

    Intensity and Ratio values for NO TEST CON Day 1 group across brain regions.

    Journal: Scientific Reports

    Article Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats

    doi: 10.1038/s41598-018-29625-x

    Figure Lengend Snippet: Intensity and Ratio values for NO TEST CON Day 1 group across brain regions.

    Article Snippet: Primary antibodies were 1:100 mouse monocolonal DARPP32 (H-3, sc-271111, lot B1815, Santa Cruz Biotechnology, Dallas, TX) and 1:100 goat polyclonal Thr34 DARPP32 (sc-21601, lot J2915, Santa Cruz Biotechnology).

    Techniques: